Monoclonal Antibody to Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) (Clone : KRTH/1076)

This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1) ; 64kDa (CK3) ; 59kDa (CK4) ; 58kDa (CK5) ; 56kDa (CK6) ; 52kDa (CK8) . Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Product Specifications

Product Name Alternative

KRT76||KRT2B||KRT2P

Gene Name

KRT76

Gene ID

51350

UniProt

Q01546

Host

Mouse

Reactivity

Human, Rat

Immunogen

Recombinant human KRT76 protein

Target Antigen

Cytokeratin, Basic (Type II or HMW) (Epithelial Marker)

Clonality

Monoclonal

Clone

KRTH/1076

Applications

IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)