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Monoclonal Antibody to Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) (Clone : KRTL/1077)

Selected Variant

36-11115-100

Size: 100 µg

This MAb recognizes the 56.5kDa (CK10) ; 50kDa (CK14) ; 50kDa (CK15) ; 48kDa (CK16) ; 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Product Specifications

Product Name Alternative

KRT77||KRT1B

Gene Name

KRT77

Gene ID

374454

UniProt

Q7Z794

Host

Mouse

Reactivity

Human, Rat

Immunogen

Recombinant human KRT77 protein

Target Antigen

Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker)

Clonality

Monoclonal

Clone

KRTL/1077

Applications

IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)

Isotype

Mouse IgG1, kappa

Price

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