Monoclonal Antibody to Ep-CAM / CD326 (Rat) (Epithelial Marker) (Clone : SPM534)

Epitope of GZ-1 and GZ-20 monoclonal antibodies are different. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.

Product Specifications

Product Name Alternative

Epcam||Tacstd1

Gene Name

Epcam

Gene ID

171577

UniProt

O55159

Host

Mouse

Reactivity

Rat

Immunogen

Rat small intestine cells

Target Antigen

Ep-CAM / CD326 (Rat) (Epithelial Marker)

Clonality

Monoclonal

Clone

SPM534

Applications

FACS, IF, WB, IP, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (5-10ul/million cells) ; Immunofluorescence (1:50-1:100) ; Western Blot (1:100-1:200) ; Immunoprecipitation (5-10ul/500ug protein lysate) ; Immunohistochemistry (Frozen & formalin-fixed) (1:100-1:200 for 30 min at RT) (Formalin/paraffin tissues REQUIRE digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C)