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Monoclonal Antibody to Human Nuclear Antigen (HNA) (Human Cell Marker) (Clone : 235-1)

This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Product Specifications

Host

Mouse

Reactivity

Human

Immunogen

Nuclei of human myeloid leukemia biopsy cells

Target Antigen

Human Nuclear Antigen (HNA) (Human Cell Marker)

Clonality

Monoclonal

Clone

235-1

Applications

FACS, IF, IP, ICC, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; , Immunoprecipitation (1-2 g/500ug protein lysate) ; , Immunocytochemistry (Acetone-fixed cells) (1-2ug/ml for 30 minutes at RT), Immunohistology (Frozen) (1-2ug/ml for 30 minutes at RT)

Isotype

Mouse IgG1, kappa

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