Monoclonal Antibody to Human Nucleolar Antigen (Marker For Human Cells) (Clone : NM95)

This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb NM95 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Product Specifications

Host

Mouse

Reactivity

Human

Immunogen

Nuclei of myeloid leukemia biopsy cells

Target Antigen

Human Nucleolar Antigen (Marker For Human Cells)

Clonality

Monoclonal

Clone

NM95

Applications

FACS, ICC, IHC, IF

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (1-2ug/million cells) . , Immunofluorescence (1-2ug/ml) ; , Immunocytochemistry (Acetone-fixed cells) (1-2ug/ml for 30 minutes at RT) ; , Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)