Monoclonal Antibody to Bromodeoxyuridine (BrdU) (Proliferation Marker) (Clone : BRD494)

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Product Specifications

Host

Mouse

Reactivity

All species

Immunogen

Bromodeoxyuridine (BrdU) conjugated to KLH

Target Antigen

Bromodeoxyuridine (BrdU) (Proliferation Marker)

Clonality

Monoclonal

Clone

BRD494

Applications

FACS, IF, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (0.5-1ug/million cells) ; Immunofluorescence (0.5-1ug/ml) ; Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) [For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC]