Monoclonal Antibody to Biotin (Vitamin B7 or Vitamin H) (Clone : Hyb-8)

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Product Specifications

Host

Mouse

Reactivity

All species

Immunogen

Biotinylated sheep immunoglobulin

Target Antigen

Biotin (Vitamin B7 or Vitamin H)

Clonality

Monoclonal

Clone

Hyb-8

Applications

FACS, IF, WB, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Confocal Microscopy; In situ Nucleic Acid Hybridization; Flow Cytometry (0.5-1ug/million cells) ; , Immunofluorescence (0.5-1ug/ml) ; Western Blot (0.5-2ug/ml) ; , Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes)