Monoclonal Antibody to Cytokeratin 5/8 (Clone : SPM268)

It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa) . Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

Product Specifications

Product Name Alternative

KRT5

Gene Name

KRT5

Gene ID

3852

UniProt

P13647

Host

Mouse

Reactivity

Human, Mouse, Rat, Hamster

Immunogen

Cytoskeletal prepaRation from HeLa cells

Target Antigen

Cytokeratin 5/8

Clonality

Monoclonal

Clone

SPM268

Applications

FACS, IF, WB, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at RT)