Monoclonal Antibody to Testosterone (Clone : 4E1G2)

This MAb is highly specific to testosterone. Its affinity constant for testosterone is ~1010M-1. In competitive binding immunoassay, it reacts with testosterone 100%, 11-beta-hydroxy testosterone 3.3%, 17-alpha-methyl testosterone

Product Specifications

Host

Mouse

Reactivity

All species

Immunogen

Testosterone 3 CMO conjugated to BSA

Target Antigen

Testosterone

Clonality

Monoclonal

Clone

4E1G2

Applications

ELISA, Radioimmnoassay, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

ELISA (For coating, order Ab without BSA) ; Radioimmnoassay (RIA) ; Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)