Monoclonal Antibody to Phosphotyrosine (P-Tyr) (Clone : PY20)

Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.

Product Specifications

Host

Mouse

Reactivity

All species

Immunogen

Phosphotyrosine conjugated to KLH

Target Antigen

Phosphotyrosine (P-Tyr)

Clonality

Monoclonal

Clone

PY20

Applications

FACS, IF, WB, IP, IHC

Purification

Affinity Chromatography

Format

Purified

Components

100 μg in 500 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (0.5-1ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Western Blotting (0.5-1ug/ml) ; Immunoprecipitation (1-2ug/500ug protein lysate) ; Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues)