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Anti-CD8b, Mouse Monoclonal Antibody (Clone: H35-17.2)

This monoclonal antibody reacts with the Mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. This monoclonal antibody identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thoμght to play a role in the process of T-cell mediated killing.

Product Specifications

Product Name Alternative

CD8b antigen; CD8 antigen beta polypeptide 1 (p37) ; CD8 beta; CD8B1; Leu2; Ly3; LYT3; P37; T lymphocyte surface glycoprotein beta chain; T-cell surface glycoprotein CD8 beta chain

Gene Name

Cd8b1 (Mouse)

Gene ID

12526

UniProt

P10300

Host

Rat

Reactivity

Mouse

Immunogen

Five day mixed leukocyte culture population, C57BL/6 anti-BALB/c

Clonality

Monoclonal

Clone

H35-17.2

Applications

Functional Assay, FACS, IF

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Functional Studies (Order Antibody without BSA & Azide) ; Flow Cytometry (0.5-1ug/million cells in 0.1ml) ; Immunofluorescence (1-2ug/ml)

Isotype

Rat IgG2b, kappa

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