Anti-PD1, Mouse Monoclonal Antibody (Clone: RMP1-14)

PD-1 is expressed on a subset of CD4-CD8-thymocytes, and on activated T and B cells. PD-1 is thoμght to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B-lymphocytes and myeloid cells. Additionally, Pdcd1mRNA is expressed in developing B-lymphocytes during the pro-B-cell stage. The RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production co-stimulated by macrophages (but not by dendritic cells and B cells) .

Product Specifications

Product Name Alternative

CD279; CD279 antigen; PD1; PDCD1; Programmed cell death 1; Programmed cell death protein 1; Systemic lupus erythematosus susceptibility 2 (SLEB2)

Gene ID

18566

UniProt

Q02242

Host

Rat

Reactivity

Mouse

Immunogen

PDCD1 transfected BHK cells

Clonality

Monoclonal

Clone

RMP1-14

Applications

ELISA, FACS, IF

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

ELISA (For coating, order Ab without BSA & Azide) ; Neutralization Studies (Order Ab without BSA & Azide) ; Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml)