Anti-Biotin (Vitamin B7 or Vitamin H) Monoclonal Antibody (Clone: BTN/2032R)

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Product Specifications

Product Name Alternative

Vitamin B7 or Vitamin H

Host

Rabbit

Reactivity

All species

Immunogen

Biotinylated sheep immunoglobulin

Clonality

Monoclonal

Clone

BTN/2032R

Applications

IHC

Components

200 μg/mL of Ab Purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

In situ Nucleic Acid Hybridization; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.)