Anti-Cytokeratin 5/8 Monoclonal Antibody (Clone: C-50)

It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa) . Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

Product Specifications

Product Name Alternative

CK5; DDD; EBS2; Epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types; K5; Keratin complex 2, basic, Gene 5; Keratin Type II Cytoskeletal 5; Keratin-5; KRT5; KRT5A; Type II Cytoskeletal 5; Type-II Keratin Kb5. CK8; CARD2; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; K

Gene Name

KRT5 & KRT8

Gene ID

3852; 3856

UniProt

P13647 ; P05787

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Cytoskeletal preparation from HeLa cells

Clonality

Monoclonal

Clone

C-50

Applications

FACS, IF, WB, IHC

Components

0.5 ml at 200 μg/mL of antibody Purified from Bioreactor Concentrate by Protein A/G chromatography. Supplied in 10mM PBS, pH 7.4 with 0.05% BSA and 0.05% sodium azide. Contact us if you require this antibody in a different format.

Storage Conditions

Store at 2 to 8°C. Antibody is stable for 24 months.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at RT)