Anti-Cytokeratin, Multi (Epithelial Marker) Monoclonal Antibody (Clone: C11)

This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Product Specifications

Gene Name

KRT4; KRT5; KRT6; KRT8; KRT10; KRT13; KRT18

Gene ID

3851; 3852; 3853; 3856; 3858; 3860; 3875

UniProt

P19013; P13647; P02538 ; P05787; P13645; P13646; P05783

Host

Mouse

Reactivity

Human, Rat, Mouse

Immunogen

Keratin-enriched preparation from cultured human A431

Clonality

Monoclonal

Clone

C11

Applications

FACS, IF, WB, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-4ug/ml) ; Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)