Anti-Cytokeratin, Multi (Epithelial Marker) Monoclonal Antibody (Clone: C11)
This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Product Specifications
Gene Name
KRT4; KRT5; KRT6; KRT8; KRT10; KRT13; KRT18
Gene ID
3851; 3852; 3853; 3856; 3858; 3860; 3875
UniProt
P19013; P13647; P02538 ; P05787; P13645; P13646; P05783
Host
Mouse
Reactivity
Human, Rat, Mouse
Immunogen
Keratin-enriched preparation from cultured human A431
Clonality
Monoclonal
Clone
C11
Applications
FACS, IF, WB, IHC
Components
200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.
Storage Conditions
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
Applications Notes
Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-4ug/ml) ; Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Isotype
Mouse IgG1, kappa
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