Anti-Proliferation Marker Monoclonal Antibody (Clone: JC1)
JC1 detects a nuclear antigen, distinct from Ki67, present in proliferating cells. It is useful as a marker of proliferation in cases such as squamous cell carcinoma where the Ki67 index cannot be determined.
Product Specifications
Host
Mouse
Reactivity
Human
Immunogen
Pokeweed-stimulated lymphocytes
Clonality
Monoclonal
Clone
JC1
Applications
WB, IF, IHC
Components
200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.
Storage Conditions
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
Applications Notes
Western Blot (1-2ug/ml) ; Immunofluorescence (1-2ug/ml) ; , Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Isotype
Mouse IgG2a, kappa
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