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Anti-E. coli O157 Monoclonal Antibody (Clone: 9.88)

This MAb shows specificity to E.coli O157 in a simple ELISA. Escherichia coliare Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms) . Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56) . TheE. colithat cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenicE. coli, which are classified into the following 4 groups according to differences in the mode of pathogenicity; enteropathogenicE. coli (EPEC), enteroinvasiveE. coli (EIEC), enterotoxigenicE. coli (ETEC) and enterohemorrhagicE. coli (EHEC) . Althoμgh the identification of pathogenicE. colirequires verification of their pathogenicity, pathogenicE. colioften have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenicE. coli.

Product Specifications

Product Name Alternative

E. coli O157

Host

Mouse

Immunogen

A crude sonicate of E. coli O157

Clonality

Monoclonal

Clone

9.88

Applications

ELISA, IF

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

ELISA (For coating use Ab at 1-5ug/ml, order Ab without BSA) ; Immunofluorescence (1-2ug/ml)

Isotype

Mouse IgM, kappa

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