Anti-CD81 / TAPA-1 Monoclonal Antibody (Clone: 1.3.3.22)

Recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1) ) . CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Product Specifications

Product Name Alternative

CD81; Target of the antiproliferative antibody 1 (TAPA-1) ; Tetraspanin-28; Tspan-28

Gene Name

CD81

Gene ID

975

UniProt

P60033

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

B-Cell line derived from a Burkitt lymphoma

Clonality

Monoclonal

Clone

1.3.3.22

Applications

Functional Assay, FACS, IF, WB, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Functional Studies (Order Ab without Azide) ; Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Western Blot (1-2ug/ml for 2 hours at RT) ; Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)