Anti-CD70 / TNFSF7 (Activated T- & B-Lymphocyte Marker) Monoclonal Antibody (Clone: BU69)

It recognizes a protein of 30kDa, identified as CD70. It is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T- and B-lymphocytes. It induces proliferation of co-stimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis. This MAb blocks the interaction between CD27 and CD70, and has been shown toinhibit T cell proliferation induced by dendritic cells.

Product Specifications

Product Name Alternative

CD27 ligand (CD27L) ; CD27LG; Ki24; Surface antigen CD70; Tumor necrosis factor (ligand) superfamily, member 7 (TNFSF7)

Gene Name

CD70

Gene ID

970

UniProt

P32970

Host

Mouse

Reactivity

Human

Immunogen

Human WM-1 (Waldenstrom s macroglobulinemia) cell line

Clonality

Monoclonal

Clone

BU69

Applications

ELISA, FACS, IF, IP, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

ELISA (For coating, order Ab without BSA) ; , Inhibits T cell proliferation induced by dendritic cells (Order Ab without Azide) ; , Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; , Immunoprecipitation (1-2ug/500ug protein lysate) ; , Immunohistochemistry (Frozen) (1-2ug/ml for 30 minutes at RT)