Anti-CD40/ TNFRSF5 / CD40L-Receptor Monoclonal Antibody (Clone: C40/2383)

CD40 is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thoμght to be an early event in Alzheimer disease pathogenesis. CD40 is expressed on B-lymphocytes, follicular dendritic cells, bone marrow-derived dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zones of secondary lymphoid organs.

Product Specifications

Product Name Alternative

B-cell surface antigen CD40; Bp50; CD40; CD40L receptor; GP39; HIGM1; IGM; IMD3; p50; TBAM; TNF receptor superfamily member 5; TNFRSF5; TRAP

Gene Name

CD40

Gene ID

958

UniProt

P25942

Host

Mouse

Reactivity

Human

Immunogen

Recombinant fragment of human CD40 protein (around aa 29-107) (exact sequence is proprietary)

Clonality

Monoclonal

Clone

C40/2383

Applications

ELISA, FACS, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

ELISA (For coating, order antibody without BSA) ; Flow Cytometry (1-2ug/million cells) ; , Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes)