Anti-PDZ and LIM Domain 1 Monoclonal Antibody (Clone: CPTC-PDLIM1-1)

CLP36, also known as PDLIM1, CLIM1 or Elfin, is a 329 amino acid cytoplasmic protein that associates with actin stress fibers at the cytoskeleton. Expressed at high levels in skeletal muscle and heart and at lower levels in colon, small intestine, spleen, lung, placenta, kidney, liver, thymus and pancreas, CLP36 functions as a cytoskeletal protein that is thoμght to act as an adaptor, bringing target proteins to the cytoskeleton. Specifically, CLP36 interacts with Clik1 (-actinin-1, thereby facilitating the association of Clik1 with actin stress fibers. CLP36 contains one PDZ domain and one LIM zinc-binding domain throμgh which it conveys its protein-protein binding capabilities. Human CLP36 shares 88% sequence similarity with its rat counterpart, sμggesting a conserved function between species.

Product Specifications

Product Name Alternative

C terminal LIM domain protein 1; carboxy terminal LIM domain protein 1; CLIM1; CLP36; Elfin; Enigma antibody; Enigma homolog antibody; hCLIM1; PDLIM1; PDZ and LIM domain 1

Gene Name

PDLIM1

Gene ID

9124

UniProt

O00151

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human full-length PDLIM1 protein

Clonality

Monoclonal

Clone

CPTC-PDLIM1-1

Applications

WB, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)