Anti-CD262 / DR5 Monoclonal Antibody (Clone: DR5/3381)

Tumor necrosis factor (TNF) is a pleiotropic cytokine whose function is mediated by two distinct cell surface receptors, designated TNF-alpha1 and TNF-alpha2, which are expressed on most cell types. TNF function is primarily mediated throμgh TNF-alpha1 signaling. Both receptors belong to the growing TNF receptor superfamily which includes Fas antigen and CD40. TNF-alpha1 contains a cytoplasmic motif, termed the death domain , that has been found to be necessary for the transduction of the apoptotic signal. The death domain is also found in several other receptors, including Fas, DR2 (or TRUNDD), DR3 (death receptor 3), DR4 and DR5. TRUNDD, DR4 and DR5 are receptors for the apoptosis-inducing cytokine TRAIL. A non-death domain-containing receptor, designated decoy receptor (DcR1 or TRID), also specifically associates with TRAIL and may play a role in cellular resistance to apoptotic stimuli.

Product Specifications

Product Name Alternative

Fas like protein; Apoptosis inducing protein TRICK2A/2B; Apoptosis inducing receptor TRAIL R2; Cytotoxic TRAIL receptor 2; Death domain containing receptor for TRAIL/Apo 2L; Death receptor 5 (DR5) ; p53 regulated DNA damage inducible cell death receptor (killer) ; TNF related apoptosis inducing ligand

Gene Name

TNFRSF10B

Gene ID

8795

UniProt

521456

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human DR5 protein fragment (around aa266-393) (exact sequence is proprietary)

Clonality

Monoclonal

Clone

DR5/3381

Applications

IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)