Anti-CD137L / 4-1BBL / TNFSF9 Monoclonal Antibody (Clone: CD137L/1547)

TNFSF9 / 4-1BBL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9 / 4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9 / 4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. It has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine is required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thoμght to be involved in T cell-tumor cell interaction.

Product Specifications

Product Name Alternative

4-1BB ligand; 4-1BBL; CD137L; ILA ligand (TNF related) ; Ly63l; Tumor necrosis factor ligand 5A (TNLG5A) ; Tumor necrosis factor ligand superfamily member 9 (TNFSF9)

Gene Name

TNFSF9

Gene ID

8744

UniProt

P41273

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human CD137L protein

Clonality

Monoclonal

Clone

CD137L/1547

Applications

FACS, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Flow Cytometry (1-2ug/million cells in 0.1ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)