Anti-Calpain 1 Monoclonal Antibody (Clone: CAPN1/1530)

Calpain 1 is an intracellular calcium-dependent protease that cleaves cytoskeletal and sub-membranous proteins. Calpains are non-lysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2+- dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Calpain 1 co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction.

Product Specifications

Product Name Alternative

Calcium dependent protease small subunit 1; Calcium-activated neutral proteinase 1; Calpain 1 large subunit; Calpain mu-type; Calpain regulatory subunit; Calpain small subunit 1; Calpain, large polypeptide L1; Calpain-1 catalytic subunit; CANP small subunit; CANP1; CANPL1; CAPN1; Cell proliferation-

Gene Name

CAPN1

Gene ID

823

UniProt

P07384

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human CAPN1 protein

Clonality

Monoclonal

Clone

CAPN1/1530

Applications

ELISA, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)