Anti-B7-H4 (Immuno-Inhibitory Protein) Monoclonal Antibody (Clone: B7H4/2652R)

T cell activation and immune function are regulated by the innate immune system throμgh positive and negative costimulatory proteins. One such protein, B7-H4 (B7-homolog 4), belongs to the B7 immunoglobulin superfamily of ligand-lymphocyte interacting proteins. Expressed primarily on the membrane of lymphoid cells, B7-H4 is an immuno-inhibitory protein that interacts with receptors on the surface of T lymphocytes, thus mediating cellular and humoral immune responses. Overexpression of the B7-H4 protein is associated with certain malignancies, including ovarian and breast cancer, as its interaction with T cells suppresses tumor-associated immunity. Current research sμggests that, similar to Mucin 16 (CA-125), B7-H4 may be a useful biomarker for the early detection of ovarian cancer.

Product Specifications

Product Name Alternative

B7 family member, H4; B7 homolog 4; B7 superfamily member 1; B7-H4; B7S1; Immune costimulatory protein B7-H4; T-cell costimulatory molecule B7x; V-set domain-containing T-cell activation inhibitor 1; VCTN1

Gene Name

VTCN1

Gene ID

79679

UniProt

Q7Z7D3

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant fragment of human B7-H4 protein (exact sequence is proprietary)

Clonality

Monoclonal

Clone

B7H4/2652R

Applications

ELISA, FACS, IF, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA) ; Flow Cytometry (1-2ug/million cells in 0.1ml) ; Immunofluorescence (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)