Anti-14-3-3E / Tryptophan 5-Monooxygenase Monoclonal Antibody (Clone: CPTC-YWHAE-1)

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, sμggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. The YWHAE gene which encodes 14-3-3E has also been identified as a possible susceptibility gene for schizophrenia.

Product Specifications

Product Name Alternative

MDS; epididymis luminal protein 2; mitochondrial import stimulation factor (MSF) L subunit; Mitochondrial import stimulation factor L subunit; MSF L; protein kinase C inhibitor protein-1

Gene Name

YWHAE

Gene ID

7531

UniProt

P62268

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human full-length YWHAE protein

Clonality

Monoclonal

Clone

CPTC-YWHAE-1

Applications

WB, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)