Anti-VEGF (Vascular Endothelial Growth Factor) Monoclonal Antibody (Clone: VG1)

This MAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF) . It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet-derived growth factor (PDGF) . There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

Product Specifications

Product Name Alternative

MVCD1; Vascular Endothelial Growth Factor A (VEGF-A) ; Vascular Permeability Factor (VPF) ; VEGFA

Gene Name

VEGFA

Gene ID

7422

UniProt

P15692

Host

Mouse

Reactivity

Human

Immunogen

Human VEGF189 recombinant protein

Clonality

Monoclonal

Clone

VG1

Applications

ELISA, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

ELISA (For coating, order Ab without BSA) ; Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 8.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)