Anti-Thymidylate Synthase (5-FU Resistance Marker) Monoclonal Antibody (Clone: TYMS/1884)

It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45) . It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drμgs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Product Specifications

Product Name Alternative

DTMP synthase, TMS, TS, TSase, TYMS protein, Tyms thymidylate synthetase

Gene Name

TYMS

Gene ID

7298

UniProt

P04818

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human thymidylate synthase protein fragment (around aa 60-174) (exact sequence is proprietary)

Clonality

Monoclonal

Clone

TYMS/1884

Applications

FACS, IF, WB, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Western Blot (1-2ug/ml) ; , Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)