Anti-Thrombomodulin / CD141 (Endothelial Cell Marker) Monoclonal Antibody (Clone: THBD/1591)

It recognizes a protein of 75kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi's sarcoma, angiosarcoma, and epithelioid hemangioendothelioma) . Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.

Product Specifications

Product Name Alternative

AHUS6; BDCA3; CD141; Fetomodulin; Thbd; THPH12; THRM; Thrombomodulin (TM)

Gene Name

THBD

Gene ID

7056

UniProt

P07204

Host

Mouse

Reactivity

Human

Immunogen

Recombinant fragment (around aa 69-194) of human Thrombomodulin (CD141) protein (exact sequence is proprietary)

Clonality

Monoclonal

Clone

THBD/1591

Applications

ELISA, WB, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA) ; Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)