Anti-TCF4 (Transcription Factor 4) Monoclonal Antibody (Clone: TCF4/1705)

Recognizes a protein of 71kDa, identified as Transcription Factor 4 (TCF4) . It is a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome.

Product Specifications

Product Name Alternative

BHLHb19; Class B basic helix-loop-helix protein 19; Immunoglobulin transcription factor 2; ITF-2; PTHS; SEF2; SL3-3 enhancer factor 2; TCF4; Transcription factor 4

Gene Name

TCF4

Gene ID

6925

UniProt

P15884

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human TCF4 protein fragment (around aa 365-671) (exact sequence is proprietary)

Clonality

Monoclonal

Clone

TCF4/1705

Applications

ELISA, WB, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

ELISA (For coating, order antibody without BSA) ; , Western Blot (1-2ug/ml) ; , Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes)