Anti-Synaptophysin (Neuroendocrine Marker) Monoclonal Antibody (Clone: SYP/3551)

This Monospecific monoclonal antibody recognizes a protein of 38kDa that is identified as synaptophysin. It is an N-glycosylated integral membrane protein found in neurons and endocrine cells. Synaptophysin contains four transmembrane domains and may function as a gap junction-like channel. This antibody identifies normal neuroendocrine cells and neuroendocrine neoplasms. Diffuse, finely granular, cytoplasmic staining is observed, which probably correlates with the distribution of the antigen within neurosecretory vesicles. Synaptophysin is an independent, broad-range marker of neural and neuroendocrine differentiation.

Product Specifications

Product Name Alternative

Major synaptic vesicle protein p38; MRX96; MRXSYP; Syn p38; Syp; SYPH

Gene Name

SYP

Gene ID

6855

UniProt

P08247

Host

Mouse

Reactivity

Human

Immunogen

Recombinant fragment (around aa 224-313) of human Synaptophysin (SYP) protein (exact sequence is proprietary)

Clonality

Monoclonal

Clone

SYP/3551

Applications

IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)