Anti-PU.1 (SPI-1) (B-Cell Marker) Monoclonal Antibody (Clone: PU1/2146)

PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.

Product Specifications

Product Name Alternative

Transcription Factor spi1; 31kDa Transforming Protein; Hematopoietic Transcription Factor PU.1; SFPI1; SPI1; SPIA; Spleen focus forming virus (SFFV) proviral integration oncogene spi1

Gene Name

SPI1

Gene ID

6688

UniProt

P17947

Host

Mouse

Reactivity

Human

Immunogen

Recombinant fragment (around aa 16-170) of human PU.1 protein (Exact sequence is proprietary)

Clonality

Monoclonal

Clone

PU1/2146

Applications

FACS, IF, WB, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; , Western Blot (1-2ug/ml) ; , Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)