Anti-SOX9 / SRY-box 9 Monoclonal Antibody (Clone: SOX9/2398)

Sox genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. SOX9 plays an important role in the normal skeletal development. It may regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.

Product Specifications

Product Name Alternative

Campomelic Dysplasia Autosomal Sex Reversal (CMD1) ; SRA1; SRXX2; SRY (sex determining region Y) box 9; SRY related HMG box gene 9; Transcription factor SOX9

Gene Name

SOX9

Gene ID

6662

UniProt

P48436

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human SOX9 protein fragment (around aa 393-508) (exact sequence is proprietary)

Clonality

Monoclonal

Clone

SOX9/2398

Applications

IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes)