Anti-SOX2 (Embryonic Stem Cell Marker) Monoclonal Antibody (Clone: rSOX2/1791)

SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. Reportedly, SOX2 is associated with aggressive phenotypes of breast, head and neck, gastric, colorectal, bladder, and small cell lung cancers. However, SOX2 is expressed in a high percentage of lung squamous cell carcinomas and has been shown to be an independent favorable prognostic marker.

Product Specifications

Product Name Alternative

ANOP3; Delta EF2a; MCOPS3 (Microphthalmia Syndromic type 3) ; SOX-2; SRY (sex determining region Y) box 2; SRY related HMG box 2; Transcription factor SOX-2; ysb

Gene Name

SOX2

Gene ID

6657

UniProt

P48431

Host

Mouse

Reactivity

Human

Immunogen

Recombinant fragment (within aa176-305) of human SOX2 protein (exact sequence is proprietary)

Clonality

Monoclonal

Clone

RSOX2/1791

Applications

IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Immunohistochemistry (Formalin-fixed; Not suitable for frozen tissues) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)