Monoclonal Antibody to Insulin (Clone: 2D11-H5)

Insulin is a secreted peptide hormone produced by the pancreas that regulates carbohydrate and lipid metabolism. Insulin is released from the pancreatic beta cells of the islets of Langerhans in response to secretagogues. Following its release, insulin quickly acts to both inhibit hepatic glucose production and stimulate peripheral blood glucose utilization. During this process cells in the liver, muscle, and fat are triggered to take up glucose and store it as glycogen, resulting in a decrease of blood glucose levels.

Product Specifications

Product Name Alternative

INS

Gene Name

INS

Gene ID

3630

UniProt

P01308

Host

Mouse

Reactivity

Human

Immunogen

Human insulin conjugated to BSA was used as immunogen to generate the insulin antibody.

Target Antigen

Insulin

Clonality

Monoclonal

Clone

2D11-H5

Applications

FACS, IF, IHC

Purification

Protein G Chromatography

Format

Purified

Components

25 μg in 50 μl/100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 minutes at RT) (No special pretreatment is required for staining of formalin/paraffin tissues.)