Anti-Prostate Specific Acid Phosphatase (PSAP) Monoclonal Antibody (Clone: SPM312)

Recognizes a protein of 52kDa, identified as prostate specific acid phosphatase (PSAP) . This enzyme catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland.PSAP is found in non-neoplastic adult and fetal prostatic glands, primary and metastatic prostatic carcinomas. It shows no staining in granulocytes, osteoclasts, parietal cells of the stomach, liver cells, renal cell or breast carcinomas.

Product Specifications

Product Name Alternative

5'-nucleotidase (5'-NT) ; Acid phosphatase prostate; ACP3; Ecto-5'-nucleotidase; Prostatic acid phosphatase (PAP) ; Prostatic acid phosphatase; Thiamine monophosphatase (TMPase)

Gene Name

ACPP

Gene ID

UniProt

P15309

Host

Mouse

Reactivity

Human

Immunogen

Prostatic acid phosphatase Purified from human seminal plasma

Clonality

Monoclonal

Clone

SPM312

Applications

FACS, IF, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)