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Anti-Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker) Monoclonal Antibody (Clone: SPM191)

Selected Variant

36-2967-100

Size: 100 µg

This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20) . KRT is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.

Product Specifications

Product Name Alternative

CK20; Cytokeratin-20; K20; KA20; Keratin 20; keratin 20, type I; Keratin type I cytoskeletal 20; Keratin-20; KRT20

Gene Name

KRT20

Gene ID

54474

UniProt

P35900

Host

Mouse

Reactivity

Human

Immunogen

Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)

Clonality

Monoclonal

Clone

SPM191

Applications

WB, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)

Isotype

Mouse IgG2b, kappa

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