Anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal Antibody (Clone: PECAM1/3527)

CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Product Specifications

Product Name Alternative

EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA'

Gene Name

PECAM1

Gene ID

5175

UniProt

P16284

Host

Mouse

Reactivity

Human

Immunogen

Recombinant fragment (around aa 625-738) of human CD31 protein (exact sequence is proprietary)

Clonality

Monoclonal

Clone

PECAM1/3527

Applications

WB, FACS, IF, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Western Blot (2-4ug/ml) ; Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; , Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)