Anti-PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Monoclonal Antibody (Clone: PC5)

Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA) . It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.

Product Specifications

Product Name Alternative

Cyclin; DNA polymerase delta auxiliary protein; Mutagen-sensitive 209 protein; PCNAR; Polymerase delta accessory protein

Gene Name

PCNA

Gene ID

5111

UniProt

P12004

Host

Mouse

Reactivity

Human, Mouse, Rat, Yeast

Immunogen

Rat PCNA/protein A fusion protein

Clonality

Monoclonal

Clone

PC5

Applications

WB, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Western Blot (1-2ug/ml) ; , Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes)