Anti-NKX2.2 (Neuroendocrine & Ewing's Sarcoma Marker) Monoclonal Antibody (Clone: NX2/1523)

Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors.

Product Specifications

Product Name Alternative

Homeobox protein NK-2 homolog B, NK2 transcription factor like protein B, NK2 transcription factor related locus 2, NKX22, Nkx2b, tinman

Gene Name

NKX2-2

Gene ID

4821

UniProt

O95096

Host

Mouse

Reactivity

Human

Immunogen

Recombinant fragment (around aa1-119) of human NKX2.2 protein (exact sequence is proprietary)

Clonality

Monoclonal

Clone

NX2/1523

Applications

IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)