Anti-MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Monoclonal Antibody (Clone: SPM493)

This MAb reacts with MUC1/EMA. It is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells. It is expressed on most secretory epithelium, including mammary gland. It is expressed abundantly in lactating mammary glands and over expressed abundantly in 90% breast carcinomas and metastases.MUC1/EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack.In immunohistochemical assays, it superbly stains routine formalin-paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Product Specifications

Product Name Alternative

Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, Epithelial Membrane Antigen, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PE

Gene Name

MUC1

Gene ID

4582

UniProt

P15941

Host

Mouse

Reactivity

Human

Immunogen

Human breast cancer cell line ZR-75 cells

Clonality

Monoclonal

Clone

SPM493

Applications

ELISA, FACS, IF, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate on Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

ELISA (For coating use Ab at 2-5ug/ml, order Ab without BSA) (Very good capturing Ab) ; Flow Cytometry (0.25-0.5ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)