Anti-MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Monoclonal Antibody (Clone: VU-4H5)

MAb VU-4H5 reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of MAb VU4H5 is APDTR as established with 'epitope fingerprinting'. VU-4H5 preferentially binds to under-glycosylated 'tumor' MUC1. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is upregulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Product Specifications

Product Name Alternative

Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, Epithelial Membrane Antigen, H23AG, KL-6, MAM6, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated

Gene Name

MUC1

Gene ID

4582

UniProt

P15941

Host

Mouse

Reactivity

Human

Immunogen

Synthetic glycosylated MUC1 60mer tandem repeat NH2- (HGVTSAPDT (GalNAc) RPAPGSTAPPAHG) 3-COOH, conjugated to bovine serum albumin

Clonality

Monoclonal

Clone

VU-4H5

Applications

WB, FACS, IF, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Western Blot (1-2ug/ml) ; Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)