Anti-CD10 (Membrane Metalloendopeptidase) Monoclonal Antibody (Clone: MME/2579)

Recognizes a 100kDa glycoprotein, identified as CD10, also known as Common Acute Lymphocytic Leukemia Antigen (CALLA) . It is a cell surface enzyme with neutral metalloendopeptidase activity, which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt s, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML) . It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.

Product Specifications

Product Name Alternative

Atriopeptidase; Common acute lymphocytic leukemia antigen (CALLA) ; Enkephalinase (EPN) ; gp100; Membrane metalloendopeptidase (MME) ; Neprilysin; Neutral endopeptidase (NEP) ; Skin fibroblast elastase (SFE)

Gene Name

MME

Gene ID

4311

UniProt

P08473

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human CD10 protein fragment (aa 583-740) (exact sequence is proprietary)

Clonality

Monoclonal

Clone

MME/2579

Applications

ELISA, WB, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA) ; Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)