Anti-MBD1 (Methyl-CpG Binding Domain Protein 1) Monoclonal Antibody (Clone: CPTC-MBD1-1)

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD) . Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. MBD1 has also been predicted to be involved in the development and progression of tumors, and it has been shown that MBD1 is significantly upregulated in pancreatic cancer tissues when compared with surrounding normal tissues. Furthermore, MBD1 may serve as a potential therapeutic target for pancreatic cancer.

Product Specifications

Product Name Alternative

CXXC-type zinc finger protein 3; CXXC3; MBD1; MECP1 COMPLEX; Methyl-CpG-binding domain protein 1 (MBD1) ; Protein containing methyl-CpG-binding domain 1 (PCM1) ; Regulator of fibroblast growth factor 2 (FGF 2) transcription (RFT)

Gene Name

MBD1

Gene ID

4152

UniProt

Q9UIS9

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human MBD1 protein

Clonality

Monoclonal

Clone

CPTC-MBD1-1

Applications

IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)