Anti-Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker) Polyclonal Antibody

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (EpCAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. EpCAM antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. EpCAM antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Product Specifications

Product Name Alternative

Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TACSTD1; TROP1; Tumor-associated Calcium Signal Transducer 1, ECS-1, Epidermal Surface Antigen 1, ESA1, FLOT2, Flotillin-2, M17S1

Gene Name

TACSTD1

Gene ID

4072

UniProt

P16422

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human TACSTD1 protein

Clonality

Polyclonal

Applications

FACS, IF, WB, IHC

Components

200 μg/mL of Ab Purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; , Western Blotting (1-2ug/ml) ; , Immunohistology (Formalin-fixed) (1-2ug/ml for 30 min at RT), (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)