Anti-Cytokeratin 4 (KRT4) Monoclonal Antibody (Clone: KRT4/2804)

Cytokeratin 4 (KRT4) is an intermediate filament protein associated with Cytokeratin 13 (KRT13) . It is expressed in suprabasal cells of non-keratinized stratified squamous epithelium of esophagus, cornea, anus, larynx, pharynx and tongue. Decreased expression of KRT4 is associated with head and neck squamous carcinoma. It is helpful in differentiation of squamous cell carcinoma of esophagus origin from that of thyroid origin.

Product Specifications

Product Name Alternative

CK4; CYK4; Cytokeratin 4; Keratin 4; Keratin type II cytoskeletal 4; KRT4

Gene Name

KRT4

Gene ID

3851

UniProt

P19013

Host

Mouse

Reactivity

Human

Immunogen

Recombinant fragment (around aa 181-292) of human KRT4 protein (exact sequence is proprietary)

Clonality

Monoclonal

Clone

KRT4/2804

Applications

FACS, IF, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)