Anti-CD103 / Integrin alpha E (T-Cell Lymphoma & Hairy Cell Leukemia Marker) Monoclonal Antibody (Clone: ITGAE/2474)

This MAb recognizes a protein of 150kDa, identified as CD103, which is the alpha-E integrin subunit of the heterodimeric alpha-E beta-7 (aEb7) integrin belonging to a small beta-7 integrin subfamily. CD103 is expressed on more than 95% of intraepithelial CD8+ cells and on 40% of mucosa-associated T cells, whereas less than 2% of resting blood lymphocytes are CD103-positive. In several malignant conditions, such as T-cell lymphomas and hairy cell leukemia (HCL), the cells express CD103. Antibody to CD103 is an extremely useful addition to the IHC panel for the diagnosis of hairy cell leukemia (HCL) .

Product Specifications

Product Name Alternative

CD103; Human mucosal lymphocyte antigen 1 (HML1 antigen) ; Integrin alpha E heavy chain; Integrin alpha E light chain; Integrin alpha E, epithelial-associated; Integrin alpha E1; Integrin alpha-IEL (intestinal intraepithelial lymphocytes) ; Integrin alpha M290; Integrin alpha-E heavy chain; Integrin

Gene Name

ITGAE

Gene ID

3682

UniProt

P38570

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human ITGAE protein fragment (exact sequence is proprietary)

Clonality

Monoclonal

Clone

ITGAE/2474

Applications

IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)