Anti-MSH6 (DNA Mismatch Repair Protein) Monoclonal Antibody (Clone: MSH6/3085)

The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes mutS and mutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. A member of the mismatch repair family, GTBP (also designated MSH6), is an MSH2-related protein that binds to DNA containing G/T mismatches. Findings sμggest that the mismatch-binding factor in human cells is composed of a heterodimer of GTBP and MSH2.

Product Specifications

Product Name Alternative

DNA mismatch repair protein Msh6; G/T mismatch-binding protein; GTBP; GTMBP; hMSH6; HNPCC5; HSAP; MSH6; mutS (E. coli) homolog 6; MutS alpha 160kDa subunit; MutS-alpha 160kDa subunit; p160; Sperm associated protein

Gene Name

MSH6

Gene ID

2956

UniProt

P52701

Host

Mouse

Reactivity

Human

Immunogen

Recombinant fragment of human MSH6 protein (around aa 374-540) (exact sequence is proprietary)

Clonality

Monoclonal

Clone

MSH6/3085

Applications

ELISA, IF, WB, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

ELISA (For coating, order Ab without BSA) ; Immunofluorescence (1-2ug/ml) ; , Western Blot (1-2ug/ml) ; , Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)