Anti-Glucose 6-Phosphate Isomerase Monoclonal Antibody (Clone: CPTC-GPI-1)

Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency.

Product Specifications

Product Name Alternative

AMF; aurocrine motility factor; EC 5.2.1.9; GNPI; Gpi1; hexose monophosphate isomerase; neuroleukin; NLK; oxoisomerase; PGI; PHI; phosphoglucose isomerase; phosphosaccharomutase; SA-36; sperm antigen 36

Gene Name

GPI

Gene ID

2821

UniProt

P06744

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human full-length GPI protein

Clonality

Monoclonal

Clone

CPTC-GPI-1

Applications

WB, IF, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Western Blot (1-2ug/ml) ; Immunofluorescence (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)