Anti-GP2 (Glycoprotein 2) / ZAP75 Monoclonal Antibody (Clone: GP2/1712)

GP2 (glycoprotein 2), also known as ZAP75, is a 537 amino acid secreted protein. It is an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. GP2 is cleaved and then released into the pancreatic duct along with exocrine secretions. GP2 binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD) . GP2 is also expressed on the apical plasma membrane of specialized microfold (M) cells among enterocytes and serves as a transcytotic receptor for mucosal antigens. M cells are considered a promising target for oral vaccination against various infectious diseases.

Product Specifications

Product Name Alternative

Glycoprotein 2 (zymogen granule membrane) ; GP2; Pancreatic zymogen granule membrane associated protein GP2; Pancreatic zymogen granule membrane protein GP-2; ZAP75

Gene Name

GP2

Gene ID

2813

UniProt

P55259

Host

Mouse

Reactivity

Human

Immunogen

Recombinant fragment of human GP2 protein (around aa 35-179) (exact sequence is proprietary)

Clonality

Monoclonal

Clone

GP2/1712

Applications

ELISA, WB, IHC

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA) ; Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)